Elabscience

SKU: E-BC-K651-M_96T-ES

NAD-Isocitrate Dehydrogenase (NAD-IDH) Activity Assay Kit, 96T

Isocitrate dehydrogenase (IDH) is one of the inverters in the tricarboxylic acid cycle and plays an important role in energy metabolism, amino acid and vitamin synthesis. The cofactors of this enzyme include two kinds of NAD+ and NADP+, which are located in different parts of the cell respectively. In eukaryotic cells, NAD-dependent isocitrate dehydrogenase mainly exists in mitochondria. Under the activation of the activator, IDH converts isocitrate into α-ketoglutaric acid. Meanwhile, NAD+ is reduced to NADH, which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The activity of NAD-IDH can be calculated by measuring the change of absorbance value at 450 nm.

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Product Specification

Basic Information

Brands:Elabscience

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Place of Origin:China

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Sensitivity:0.84 U/L

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Shelf Life:12 months

Storage:This product can be stored at -20°C for 12 months with shading light.

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Detection Type:Colorimetric method

Detection Range: 0.84-50 U/L

Research Areas:Tricarboxylic Acid（TCA）Cycle

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Target Synonym:NAD-IDH

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Result Type:Enzyme Activity

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Assay Time:50 min

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Detection Instrument :Microplate reader

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