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SKU: 211-500
Human Endothelial Cell Media
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Human[/caption]
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\n Human[/caption]
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Media were optimized for the robust growth and the maintenance of phenotypic and functional characteristics of Endothelial Cells in culture. Media were designed with attention to detail that ensures the consistency of cell health, viability, performance, physiology, morphology and function.
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\nUse with:
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- Human Aortic Endothelial Cells: HAOEC \n
- Human Femoral Artery Endothelial Cells: HFAEC \n
- Human Pulmonary Artery Endothelial Cells: HPAEC \n
- Human Umbilical Vein Endothelial Cells: HUVEC \n
EGM v2 (Cat# 213-500) supports continuous, robust growth
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HUVEC were seeded to T25 flasks, passaged repeatedly, and counted for cell number.
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1803 HUVEC EGM2 growth curve v2[/caption]
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1803 HAOEC CD31 staining[/caption]
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\nSustained expression of endothelial cell markers
\nGrowth of primary Human Aortic Endothelialial Cells (HAOEC) in EGM (Cat# 211-500) maintains CD31 expression.
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\n[caption id="attachment_5391" align="alignnone" width="300"] 1803 HAOEC CD31 staining[/caption]
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\nFollowing treatment of TNF-alpha, Human Coronary Artery Endothelial cells (HCAEC) upregulate VCAM-1 (right). Untreated (left)
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\n[caption id="attachment_5392" align="alignnone" width="300"]
1803 HCAEC+TNFa-VCAM 1 staining v2[/caption]
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\nFor 10 days, cells were stimulated with VEGF or VEGF in the presence of the angiogenesis inhibitor suramin.
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Transfect primary endothelial cells with Cytofect Transfection Kits
\nConvenient, non-toxic transfection of HAOEC with GFP plasmid.
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\nClient Testimonial
\nAccording to Sara Lynn Farwell, Ph.D. Candidate, Cell & Molecular Bio. at Lehigh University: “CAI’s Endothelial Cell Growth Medium is reliable for our experiments, as well as general culturing. There must be magic in their media, because when we had problems with other formulations, the CAI product fixed the issues!”
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\nNote that endothelial Cell Defined Medium is designed for maintaining the monolayer of endothelial cells at 100% confluent culture. It does not contain the growth factors VEGF, FGF or PDGF, nor does it contain serum, but is instead supplemented with defined components to allow metabolic and growth factor studies without interference of extraneous factors.
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