SKU: 017DK

Human Cardiomyocyte Differentiation Media

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From Skin Cells to Beating Heart Cells  Dermal fibroblasts (HDF) isolated from human skin, reprogrammed to pluripotent stem cells (HiPSC), then differentiated to cardiomyocytes (i-HCm). (a) In vitro-cultured i-HCm immunofluorescently stained for Alpha Actinin (b) Troponin, and nuclei counter-stained for DAPI. (c) Pulsating heart tissue spheroid (organoid) constructed with Cyfuse Regenova Bio 3D Printer, incorporating (left) neonatal or (right) adult i-HCm & primary cardiac cells. Printed i-HCm derived from adult iPSC exhibit strong beating at 72 pulses per minute while neonatal i-HCm demonstrate weaker, faster beat rate. (d): Monolayer-cultured i-HCm beat spontaneously in rhythm.
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Medium carefully optimized for differentiating Human Induced Pluripotent Stem Cells into iPSC-Derived Cardiomyocytes and maintaining i-HCm.  Attention to detail ensures ideal cell health, viability, performance, physiology, morphology, consistency and data.
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